Hyphascope is an open-source, low-cost, high-resolution imaging device designed for capturing detailed images of hyphae in soil. The system utilizes a digital microscope camera (DMC) with 600× magnification to image soil profiles within a volume of 70 × 210 × 1.5 mm. It supports image resolutions of 640 × 480, 1280 × 960, and 1600 × 1200 pixels, corresponding to 1.30 μm/px, 0.65 μm/px, and 0.52 μm/px, respectively. The repository provides Python scripts for operating the device, including functions for camera movement, focus adjustment, and automated imaging sessions. Its ability to monitor soil fungal structures makes it valuable for agricultural applications such as assessing soil health, studying mycorrhizal associations, and evaluating the impact of farming practices on soil microbiota.